| Congresso Brasileiro de Microbiologia 2023 | Resumo: 362-2 | ||||
Resumo:The use of herbicide in Brazilian crops is an usual practice for optimizing the production of sugar cane which makes Brazil the second market worldwide for ethanol production. As a side effect, several socio-environmental problems may be caused by the use of pesticides, and bioremediation is considered one of the greatest strategies aiming to remove these xenobiotics from the soil. In this scene, the recovery of representative adapted microbes from contaminated sites helps with good results. The goal of this study was to analyze the potential of degrading ametryn of B. safensis C1C5502-1 isolated from sugarcane crop soil. For this, a microcosm test using sandy soil was performed in four different conditions involving the addition of 15 μL of glycerol, 500 μL of biosurfactant (concentration of 0,1g/mL), and 100μL of ametryn (from a solution of 500 mg/L of ametryn (m/v)) for 30 days. Four groups were established: 1) ametryn and B. safensis; 2) glycerol, ametryn, and B. safensis; 3) biosurfactant, ametryn, and B. safensis; 4) glycerol, biosurfactant, ametryn, and B. safensis. Cellular concentration was carried out on days 5, 10, 15, and 30 using the pour plate technique in nutrient agar. Statistical analysis of the quantifications was performed by using the software GraphPad Prism 7.00 by 2way ANOVA methodology and Bonferroni's multiple comparisons test. Samples with adjuvants and those with only ametryn and bacteria were compared at all analysis points. Results revealed that the application of glycerol and biosurfactant reduced the expected initial stress of B. safensis C1C5502-1 and statistical differences were not observed among the samples without glycerol and/or biosurfactant compared to the samples with the presence of the adjuvants. Thus, the bacteria's efficiency in using ametryn as a carbon source does not significantly improve with the use of adjuvants. Therefore, the results show that B. safensis C1C5502-1 possesses great potential to be applied in the removal of ametryn without additional costs. Palavras-chave: Bioremediation, Low-cost treatments, Ametryn, B. safensis Agência de fomento:CAPES; CNPq | |||||